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TCESBM Bootcamp

Summer Boot Camp

Computational Biology Workshop 1: Single-cell RNA Sequencing Analysis using Seurat

Presented by

Fahd Qadir, PhD

Postdoctoral Fellow

Dr. Franck Mauvais-Jarvis Lab

Section of Endocrinology and Metabolism

August 10 & 11, 2023 (Thu & Fri), 1-4pm

SOM (Hutchinson) Room 4001

Workshop outline:

Day 1:

  • Understanding the concept of scRNAseq and its applications

  • Experimental design

  • Comparison of library preparation and sequencing platforms

  • Overview of single-cell analysis using Seurat

  • Installing R and basic syntax

 

Day 2:

  • Data loading/QC/filtering

  • Normalization using VST and SC Transform

  • Multidimensional reduction and cell clustering- Differential testing framewor

  • Visualization

  • Pitfalls, caveats, and future topics

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